Finding Sequences for over 270 Orphan Enzymes
Identifieur interne : 000100 ( Main/Exploration ); précédent : 000099; suivant : 000101Finding Sequences for over 270 Orphan Enzymes
Auteurs : Alexander G. Shearer [États-Unis] ; Tomer Altman [États-Unis] ; Christine D. Rhee [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
Despite advances in sequencing technology, there are still significant numbers of well-characterized enzymatic activities for which there are no known associated sequences. These ‘orphan enzymes’ represent glaring holes in our biological understanding, and it is a top priority to reunite them with their coding sequences. Here we report a methodology for resolving orphan enzymes through a combination of database search and literature review. Using this method we were able to reconnect over 270 orphan enzymes with their corresponding sequence. This success points toward how we can systematically eliminate the remaining orphan enzymes and prevent the introduction of future orphan enzymes.
Url:
DOI: 10.1371/journal.pone.0097250
PubMed: 24826896
PubMed Central: 4020792
Affiliations:
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Rhee</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Clover Collective, Mountain View, California, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Clover Collective, Mountain View, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Despite advances in sequencing technology, there are still significant numbers of well-characterized enzymatic activities for which there are no known associated sequences. These ‘orphan enzymes’ represent glaring holes in our biological understanding, and it is a top priority to reunite them with their coding sequences. Here we report a methodology for resolving orphan enzymes through a combination of database search and literature review. Using this method we were able to reconnect over 270 orphan enzymes with their corresponding sequence. 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Alexander G" uniqKey="Shearer A" first="Alexander G." last="Shearer">Alexander G. Shearer</name></region><name sortKey="Altman, Tomer" sort="Altman, Tomer" uniqKey="Altman T" first="Tomer" last="Altman">Tomer Altman</name><name sortKey="Rhee, Christine D" sort="Rhee, Christine D" uniqKey="Rhee C" first="Christine D." last="Rhee">Christine D. Rhee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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